The SNPsig VariPLEX SARS-CoV-2 is a CE marked, in vitro diagnostic, real-time-reverse transcriptase PCR (Real-Time-PCR) multiplex assay intended for the allelic discrimination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus variants of concern 20I/501Y.V1, 20H/501Y.V2, 20J/501Y.V3 and 20C/S.452R, as well as biologically significant mutations N501Y and E484K. The multiplex assay provides rapid screening of individuals suspected of SARS-CoV-2 variants of concern or as part of wider surveillance of biological significant mutations.